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nrk52e, a rat normal renal proximal tubular epithelial cell line  (BioResource International Inc)

 
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    BioResource International Inc nrk52e, a rat normal renal proximal tubular epithelial cell line
    MTT assay of <t>NRK52E-</t> and RMC cells affected by testosterone, bicalutamide, and R1881 in 25 mM hyperglycemic medium. NRK52E cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 5% bovine serum and 0.4% PBS overnight until adhered. Finally, the medium was replaced fresh with serum free medium. RMC cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 25 mM glucose, 15% FBS, 0.4% PBS, and 0.8% G418 overnight until adhered. Finally, the medium was replaced fresh with 2% FBS. At final step, the two cells were simultaneously treated as indicated. ( a ) NRK52E + testosterone (1~10 3 nM), ( b ) NRK52E + bicalutamide (3.75~60 μM), ( c ) RMC + testosterone (1~10 3 nM), ( d ) RMC + R1881 (1~10 3 nM), and ( e ) RMC + bicalutamide, and ( f ) RMC + bicalutamide + testosterone for duration as indicated. Experiment was performed in triplicate
    Nrk52e, A Rat Normal Renal Proximal Tubular Epithelial Cell Line, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nrk52e%2C+a+rat+normal+renal+proximal+tubular+epithelial+cell+line/nrk+52e+cells/pmc06399217-52-0-21
    Average 90 stars, based on 1 article reviews
    nrk52e, a rat normal renal proximal tubular epithelial cell line - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Renal Damaging Effect Elicited by Bicalutamide Therapy Uncovered Multiple Action Mechanisms As Evidenced by the Cell Model"

    Article Title: Renal Damaging Effect Elicited by Bicalutamide Therapy Uncovered Multiple Action Mechanisms As Evidenced by the Cell Model

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-39533-3

    MTT assay of NRK52E- and RMC cells affected by testosterone, bicalutamide, and R1881 in 25 mM hyperglycemic medium. NRK52E cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 5% bovine serum and 0.4% PBS overnight until adhered. Finally, the medium was replaced fresh with serum free medium. RMC cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 25 mM glucose, 15% FBS, 0.4% PBS, and 0.8% G418 overnight until adhered. Finally, the medium was replaced fresh with 2% FBS. At final step, the two cells were simultaneously treated as indicated. ( a ) NRK52E + testosterone (1~10 3 nM), ( b ) NRK52E + bicalutamide (3.75~60 μM), ( c ) RMC + testosterone (1~10 3 nM), ( d ) RMC + R1881 (1~10 3 nM), and ( e ) RMC + bicalutamide, and ( f ) RMC + bicalutamide + testosterone for duration as indicated. Experiment was performed in triplicate
    Figure Legend Snippet: MTT assay of NRK52E- and RMC cells affected by testosterone, bicalutamide, and R1881 in 25 mM hyperglycemic medium. NRK52E cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 5% bovine serum and 0.4% PBS overnight until adhered. Finally, the medium was replaced fresh with serum free medium. RMC cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 25 mM glucose, 15% FBS, 0.4% PBS, and 0.8% G418 overnight until adhered. Finally, the medium was replaced fresh with 2% FBS. At final step, the two cells were simultaneously treated as indicated. ( a ) NRK52E + testosterone (1~10 3 nM), ( b ) NRK52E + bicalutamide (3.75~60 μM), ( c ) RMC + testosterone (1~10 3 nM), ( d ) RMC + R1881 (1~10 3 nM), and ( e ) RMC + bicalutamide, and ( f ) RMC + bicalutamide + testosterone for duration as indicated. Experiment was performed in triplicate

    Techniques Used: MTT Assay, Incubation

    Related Articles

    MTT Assay:

    Article Title: Renal Damaging Effect Elicited by Bicalutamide Therapy Uncovered Multiple Action Mechanisms As Evidenced by the Cell Model
    Article Snippet: NRK52E, a rat normal renal proximal tubular epithelial cell line; and RMC, the rat mesangial cell line, were provided by the Bioresource Collection & Research Center (BCRC; Hsinchu, Taiwan).

    Incubation:

    Article Title: Renal Damaging Effect Elicited by Bicalutamide Therapy Uncovered Multiple Action Mechanisms As Evidenced by the Cell Model
    Article Snippet: NRK52E, a rat normal renal proximal tubular epithelial cell line; and RMC, the rat mesangial cell line, were provided by the Bioresource Collection & Research Center (BCRC; Hsinchu, Taiwan).



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    nrk52e, a rat normal renal proximal tubular epithelial cell line  (BioResource International Inc)
    90
    BioResource International Inc nrk52e, a rat normal renal proximal tubular epithelial cell line
    MTT assay of <t>NRK52E-</t> and RMC cells affected by testosterone, bicalutamide, and R1881 in 25 mM hyperglycemic medium. NRK52E cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 5% bovine serum and 0.4% PBS overnight until adhered. Finally, the medium was replaced fresh with serum free medium. RMC cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 25 mM glucose, 15% FBS, 0.4% PBS, and 0.8% G418 overnight until adhered. Finally, the medium was replaced fresh with 2% FBS. At final step, the two cells were simultaneously treated as indicated. ( a ) NRK52E + testosterone (1~10 3 nM), ( b ) NRK52E + bicalutamide (3.75~60 μM), ( c ) RMC + testosterone (1~10 3 nM), ( d ) RMC + R1881 (1~10 3 nM), and ( e ) RMC + bicalutamide, and ( f ) RMC + bicalutamide + testosterone for duration as indicated. Experiment was performed in triplicate
    Nrk52e, A Rat Normal Renal Proximal Tubular Epithelial Cell Line, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nrk52e%2C+a+rat+normal+renal+proximal+tubular+epithelial+cell+line/nrk+52e+cells/pmc06399217-52-0-21
    Average 90 stars, based on 1 article reviews
    nrk52e, a rat normal renal proximal tubular epithelial cell line - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    MTT assay of NRK52E- and RMC cells affected by testosterone, bicalutamide, and R1881 in 25 mM hyperglycemic medium. NRK52E cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 5% bovine serum and 0.4% PBS overnight until adhered. Finally, the medium was replaced fresh with serum free medium. RMC cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 25 mM glucose, 15% FBS, 0.4% PBS, and 0.8% G418 overnight until adhered. Finally, the medium was replaced fresh with 2% FBS. At final step, the two cells were simultaneously treated as indicated. ( a ) NRK52E + testosterone (1~10 3 nM), ( b ) NRK52E + bicalutamide (3.75~60 μM), ( c ) RMC + testosterone (1~10 3 nM), ( d ) RMC + R1881 (1~10 3 nM), and ( e ) RMC + bicalutamide, and ( f ) RMC + bicalutamide + testosterone for duration as indicated. Experiment was performed in triplicate

    Journal: Scientific Reports

    Article Title: Renal Damaging Effect Elicited by Bicalutamide Therapy Uncovered Multiple Action Mechanisms As Evidenced by the Cell Model

    doi: 10.1038/s41598-019-39533-3

    Figure Lengend Snippet: MTT assay of NRK52E- and RMC cells affected by testosterone, bicalutamide, and R1881 in 25 mM hyperglycemic medium. NRK52E cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 5% bovine serum and 0.4% PBS overnight until adhered. Finally, the medium was replaced fresh with serum free medium. RMC cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 25 mM glucose, 15% FBS, 0.4% PBS, and 0.8% G418 overnight until adhered. Finally, the medium was replaced fresh with 2% FBS. At final step, the two cells were simultaneously treated as indicated. ( a ) NRK52E + testosterone (1~10 3 nM), ( b ) NRK52E + bicalutamide (3.75~60 μM), ( c ) RMC + testosterone (1~10 3 nM), ( d ) RMC + R1881 (1~10 3 nM), and ( e ) RMC + bicalutamide, and ( f ) RMC + bicalutamide + testosterone for duration as indicated. Experiment was performed in triplicate

    Article Snippet: NRK52E, a rat normal renal proximal tubular epithelial cell line; and RMC, the rat mesangial cell line, were provided by the Bioresource Collection & Research Center (BCRC; Hsinchu, Taiwan).

    Techniques: MTT Assay, Incubation